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Oligodeoxynucleotide synthesis today is virtually always carried out on a solid support Initially, the polymeric solid supports were polystyrene cross-linked with divinylbenzene, but controlled-pore glass beads (CPG beads) with de ned porosities are now used in preference The 3 -hydroxyl of the rst protected deoxynucleotide is precoupled to the solid support and then solid phase, multistage oligodeoxynucleotide synthesis may commence in the 3 5 direction, in direct analogy to SPPS (Figure 25) (a) The rst protected deoxynucleotide attached to the solid support is protected on the 5 hydroxyl group with dimethoxytrityl (DMT) Deprotection with trichloroacetic acid (TCA) results in a free 5 -hydroxyl group and the release of the orange coloured DMT cation (detected as a measure of the ef ciency of deprotection) (b) A 5 -DMT-protected deoxynucleotide with attached 3 -phosphoramidite (Figure 2.

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19a) a local phase transformation] a gauge field must be introduced that is coupled to baryon number This field has the same group structure as for the photon, so it is not difficult t o determine its properties For example, a Coulomb s law should result for a force acting between two objects that is proportional to the product of their baryonic charges Since baryonic charges are generally not in the same ratio as the masses for two objects, this would have observable consequences [see Okun (1982) for a discussion where it is concluded that if baryonic photons exist their coupling to baryonic matter is < the strength of photon coupling to electrical charges] There i s little substantial evidence for such effects, so we conclude that the gauging of baryon number is not useful.

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Purpose: Selects one of a series of results based on an index value. Syntax: Choose (expression; result0 [; result1; result2] . . .) where the result of an expression yields a number (between 0 and the number of the last result specified) that indexes into the result list that follows, and result is one or more results. Choose can return text, a number, a date, a time, or a container.

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6) or H-phosphonate group is then activated for coupling to the 5 -hydroxyl group of the 3 -solid-support-bound deoxynucleotide by means of a coupling reagent known as tetrazole (c) Iodine is introduced to oxidise the diester link formed from coupling to a complete phosphodiester link (with P(V) oxidation state) Diester links formed with phosphoramidites or H phosphonate are unstable (due to the P(III) oxidation state) (d) Acetic anhydride is then added to block or cap any unreacted 5 -hydroxyl groups so that sequence truncations are not incorporated in later steps (Figure 27) (e) With a phosphodiester link formed and 5 -hydroxyl capping performed, the sequential process of 5 -hydroxyl DMT deprotection and coupling of 5 -DMT-deoxynucleotide-3 phosphoramidites, then iodine oxidation and capping again of unreacted 5 -hydroxyl groups, can take place in a repetitive, iterative manner until the desired full length oligoor polydeoxynucleotide is prepared.

The entire oligo- or polydeoxynucleotide is then detached from the solid support and deprotected to complete the synthesis After synthesis of the oligo- or polydeoxynucleotide.

Similar considerations argue against gauging lepton number, isotopic spin, or S U ( 3 ) flavor symmetries..

232 F Garnier, R H Donnay, and J E Dubois, J Chern Soc, Chern Cornrnun 829 (1971) 233 A Modro, G H Schmid, and K Yates, J Org Chern 44,4221 (1979) 234 1 Roberts and G E Kimball, J Arn Chern Soc 59,947 (1937) 235 E Bienvenue-Goetz and J-E Dubois, Tetrahedron 34, 2021 (1978) 236 B Galland, E M Evleth, and M-F Ruasse, J Chern Soc, Chern Cornrnun 898 (1990) 237 T P Hamilton and H F Schaefer, III, J Arn Chern Soc 112, 8260 (1990) 238 S Yamabe and T Minato, Bull Chern Soc Jpn 66,3339 (1993) 239 G A Olah and A M White, J Arn Chern Soc 91,5801 (1969) 240 G A Olah and T R Hockswender, Jr, J Arn Chern Soc 96,3574 (1974) 241 G A Olah, P W Westerman, E G Melby, and Y K Mo, J Arn Chern Soc.

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